Cationic lipid nanoparticle having high transfection efficiency and preparation method therefor
AssigneesShuwen TONG, Beijing D-Nano Pharma Co., Ltd
InventorShuwen TONG
Life of this patent
Estimating the term…
Abstract
A nucleic acid-loaded calcium-containing cationic lipid nanoparticle, comprising a cationic lipid, a neutral lipid, a PEGylated lipid, and cholesterol and/or a cholesterol ester. The cationic lipid nanoparticle can be used for preparing a gene-based drug for local injection into the body or a nucleic acid vaccine for local or systemic injection into the body.
Claims22
A nucleic acid-loaded calcium-containing cationic lipid nanoparticle, wherein
- the cationic lipid nanoparticle comprises cationic lipid, neutral lipid, PEGylated lipid, and cholesterol and/or cholesterol ester;
- the cationic lipid nanoparticle contains calcium ions, and anions corresponding to the calcium ions are not phosphate radicals, hydrogen phosphate radicals, and dihydrogen phosphate radicals;
- and
- the cationic lipid nanoparticle is free of non-PEG-group-modified negatively charged lipid.
A calcium-containing cationic lipid nanoparticle composition, wherein the calcium-containing cationic lipid nanoparticle composition is prepared by mixing calcium-containing cationic lipid nanoparticles with nucleic acid-loaded cationic lipid nanoparticles, and
- optionally after mixing, adjusting the pH value to neutrality.
22-26. (canceled)
Dependencies are inferred from the claim text.