Reversible loading of proteins in the lumen of extracellular vesicles

Abstract

The present invention relates to fusion polypeptides comprising a sub-membrane targeting domain, a protein of interest or a functionally or structurally active fragment thereof, and a peptide interacting with the Endosomal Sorting Complexes Required for Transport (ESCRT) cellular machinery, and the use of said fusion polypeptides in methods of targeting a protein of interest in the lumen of an extracellular vesicle. The present invention also relates to extracellular vesicles comprising said fusion polypeptides, and their use for treating or preventing diseases.

Dependencies are inferred from the claim text.