Medium and device for proteomic sample preparation

Abstract

The invention provides a medium. The medium comprises a fibrous matrix and silica microparticles immobilized in the fibrous matrix. The fibrous matrix may comprise glass fibers, polyolefin fibers, polyvinylidene fluoride (PVDF) fibers, polytetrafluoroethylene (PTFE) fibers, polypropylene fibers, polyethylene fibers, aramid fibers, natural cellulosic fibers, or a combination thereof. A composition comprising the medium is provided. A device comprising the medium or the composition is also provided. Further provided are methods for preparing a proteomic sample from a biological sample on the medium. The biological sample comprises cells or proteins. The method may comprise a treating step, a digesting step, a reducing and alkylating step, and an eluting step. All of these steps may be performed on the medium. The proteomic sample comprises the eluted peptides. The proteomic sample may be desalted. The peptides prepared from the biological sample may be suitable for qualitative or quantitative analysis, for example, mass spectrometric analysis.

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