Cytosine deaminase and use thereof in base editing
OwnerCaixia GAO, Qiupeng LIN +2inventors
InventorsCaixia GAO, Qiupeng LIN, Jiaying HUANG, Kevin T. ZHAO
Life of this patent
Estimating the term…
Abstract
The present invention relates to the field of genetic engineering. Specifically, the present invention relates to cytosine deaminase and use thereof in base editing. More specifically, the present invention relates to a base editing system based on a newly identified cytosine deaminase, a method for base editing a target sequence in the genome of an organism (e.g., a plant) using the base editing system, and a genetically modified organism (e.g., a plant) produced by the method and progenies thereof.
Claims92
A cytosine deaminase, wherein the cytosine deaminase is capable of deaminating the cytosine base of deoxycytidine in DNA.
A protein clustering method, comprising:
- (1) obtaining the sequences of a plurality of candidate proteins from a database;
- (2) predicting the three-dimensional structure of each of the plurality of candidate proteins using a protein prediction program;
- (3) performing multiple structural alignment on the three-dimensional structures of the plurality of candidate proteins using a scoring function, thereby obtaining a structural similarity matrix;
- (4) clustering the plurality of candidate proteins based on the structural similarity matrix using a phylogenetic tree construction method.
Dependencies are inferred from the claim text.