Neisseria gonorrhoeae detection kit and neisseria gonorrhoeae detection method

A kit for detecting Neisseria gonorrhoeae in a sample, comprising:

• a capture antibody for capturing a Neisseria gonorrhoeae-derived antigen in the sample;
• and
• a detection antibody having a detection label for labeling the Neisseria gonorrhoeae-derived antigen in the sample,
• wherein Neisseria gonorrhoeae is detected via an immune reaction between the Neisseria gonorrhoeae-derived antigen in the sample, the capture antibody, and the detection antibody to form a structure sandwiching the Neisseria gonorrhoeae-derived antigen,
• wherein the Neisseria gonorrhoeae-derived antigen is a ribosomal protein L7/L12 of Neisseria gonorrhoeae,
• wherein one of the capture antibody and the detection antibody is a first monoclonal antibody or its fragment or a derivative thereof, which causes an antigen-antibody reaction with an epitope containing one or more amino acid residues selected from the 2nd to 14th amino acid residues from the N-terminal of the amino acid sequence of Neisseria gonorrhoeae L7/L12 shown in SEQ ID NO:1,
• wherein the other of the capture antibody and the detection antibody is a second monoclonal antibody or its fragment or a derivative thereof, which causes an antigen-antibody reaction with an epitope containing one or more amino acid residues selected from the 102nd to 123rd amino acid residues from the N-terminal of the amino acid sequence of Neisseria gonorrhoeae L7/L12 shown in SEQ ID NO:1.

A kit for detecting Neisseria gonorrhoeae in a sample, comprising:

• a capture antibody for capturing a Neisseria gonorrhoeae-derived antigen in the sample;
• and
• detection antibody having a detection label for labeling the Neisseria gonorrhoeae-derived antigen in the sample,
• wherein Neisseria gonorrhoeae is detected via an immune reaction between the capture antibody, and the detection antibody to form a structure sandwiching the Neisseria gonorrhoeae-derived antigen,
• wherein the Neisseria gonorrhoeae-derived antigen is a ribosomal protein L7/L12 of Neisseria gonorrhoeae, and
• wherein the capture antibody and the detection antibody are selected from:
• (1) a combination where the capture antibody is (a) or (b) below, and the labeling antibody is (c) below;
• or
• (2) a combination where the labeling antibody is (a) or (b) below, and the capture antibody is (c) below,
• (a) An antibody comprising:
• as the amino acid sequence of a heavy chain variable region, an amino acid sequence having a homology of 80% or more to the amino acid sequence of SEQ ID NO:11, and,
• as the amino acid sequence of a light chain variable region, an amino acid sequence having a homology of 80% or more to the amino acid sequence of SEQ ID NO:
• 12,
• (b) An antibody comprising:
• as the amino acid sequence of a heavy chain variable region, an amino acid sequence having a homology of 80% or more to the amino acid sequence of SEQ ID NO:9, and,
• as the amino acid sequence of a light chain variable region, an amino acid sequence having a homology of 80% or more to the amino acid sequence of SEQ ID NO:10,
• (c) An antibody comprising:
• as the amino acid sequence of a heavy chain variable region, an amino acid sequence having a homology of 80% or more to the amino acid sequence of SEQ ID NO:7, and,
• as the amino acid sequence of a light chain variable region, an amino acid sequence having a homology of 80% or more to the amino acid sequence of SEQ ID NO:8.