Reagent combination and method for detecting target immunosuppressant in whole blood without centrifugation

A method for detecting a target immunosuppressant in a whole blood, the method further comprises:

• providing a signal detection reagent and a magnetic carrier coated with a first antibody, wherein the signal detection reagent comprises a signal generator connected to a second antibody, the first antibody can specifically recognize and bind a target molecule in a sample to be detected to form a complex of target molecule-first antibody, the target molecule in the complex induces the first antibody to form at least one new conformational epitope, and the second antibody can specifically bind to the at least one new conformational epitope;
• using a whole blood treatment agent to treat the whole blood to be detected to obtain a treated sample to be detected;
• contacting the treated sample to be detected with the magnetic carrier coated with the first antibody to obtain a complex of target immunosuppressant-first antibody-magnetic carrier;
• contacting the complex of target immunosuppressant-first antibody-magnetic carrier with the signal detection reagent to obtain a complex of target immunosuppressant-first antibody-magnetic carrier-second antibody-signal generator;
• and
• using an immunoassay method for performing the signal detection reagent on the complex of target immunosuppressant-first antibody-magnetic carrier-second antibody-signal generator to detect the target immunosuppressant in the whole blood;
• wherein the whole blood treatment agent comprises butanol and optionally saponin or ascorbic acid.