Method for producing sesaminol or sesaminol glucoside

A method for generating sesaminol or sesaminol glucosides, comprising the steps of:

• in a host cell, an enzyme from a non-human transformed cell is reacted with substrate sesaminol glycoside having at least one glucosidic bond, and catalyzing hydrolysis of at least one glucosidic bond;
• wherein a polynucleotide is introduced into the non-human transformed cell, and the polynucleotide is selected from the group consisting of (1) to (5):
• (1) a polynucleotide encoding a protein that comprises an amino acid sequence of SEQ ID NO:
• 1;
• (2) a polynucleotide encoding a protein that comprises an amino acid sequence derived by deletion, substitution, insertion and/or addition of one or more amino acids in the amino acid sequence of SEQ ID NO:
• 1 and has an activity of catalyzing the hydrolysis of at least one glucosidic bond;
• (3) a polynucleotide encoding a protein that comprises an amino acid sequence having more than 60% sequence identity to the amino acid sequence of SEQ ID NO:
• 1 and has an activity of catalyzing the hydrolysis of at least one glucosidic bond;
• (4) a polynucleotide encoding a protein that has an activity of catalyzing the hydrolysis of at least one glucosidic bond, which hybridizes with a polynucleotide comprising a complementary base sequence at a highly demanding condition, wherein the complementary base sequence is complementary to the polynucleotide encoding the protein comprising the amino acid sequence of SEQ ID NO:
• 1;
• and (5) a polynucleotide comprising a base sequence of SEQ ID NO:2.