L-threonine transaldolases and uses thereof
AssigneeUniversity of Delaware
InventorsAditya Kunjapur, Michaela Jones, Neil Butler, Sean Wirt
Life of this patent
Estimating the term…
Abstract
The invention provides a method for producing in vitro a beta-hydroxy non-standard amino acid (0-OH-nsAA). The in vitro method comprises incubating L-threonine, an aldehyde and an L-threonine transaldolase (TTA). Also provided is a method for producing a beta-hydroxy non-standard amino acid (0-OH-nsAA) by recombinant cells, comprising expressing a heterologous L-threonine transaldolase (TTA) by the recombinant cells, and growing the recombinant cells in a medium. The medium comprises L-threonine and an aldehyde.
Claims21
A method for producing in vitro a beta-hydroxy non-standard amino acid (β-OH-nsAA), comprising incubating L-threonine, an aldehyde and an L-threonine transaldolase (TTA), wherein the TTA comprises an amino acid sequence having at least 90% identity to an amino acid sequence selected from the group consisting of SEQ IDs:
- 1-29, whereby a beta-hydroxy non-standard amino acid (β-OH-nsAA) is produced.
A method for producing a beta-hydroxy non-standard amino acid (μ-OH-nsAA) by recombinant cells, comprising:
- (a) expressing a heterologous L-threonine transaldolase (TTA) by the recombinant cells, wherein the TTA comprises an amino acid sequence having at least 90% identity to an amino acid sequence of a protein selected from the group consisting of SEQ ID NOs:
- 1-29;
- and
- (b) growing the recombinant cells in a medium, wherein the medium comprises L-threonine and an aldehyde, whereby a beta-hydroxy non-standard amino acid (β-OH-nsAA) is produced by the recombinant cells from the L-threonine and the aldehyde.
21-25. (canceled)
Dependencies are inferred from the claim text.