Method for producing methacrylic acid
AssigneeRiken
InventorsTomokazu SHIRAI, Yutaro MORI
Life of this patent
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Abstract
A method for producing methacrylic acid, comprising: decarboxylating mesaconic acid or an isomer thereof in the presence of protocatechuate decarboxylase.
Claims17
A method for producing methacrylic acid, comprising:
- decarboxylating mesaconic acid or an isomer thereof in the presence of protocatechuate decarboxylase.
A protocatechuate decarboxylase in which an amino acid at position 327 of an amino acid sequence represented by SEQ ID NO:
- 2 or an amino acid corresponding to the site is modified to methionine, phenylalanine, asparagine, isoleucine, glutamine, serine, valine, threonine, tyrosine, or leucine, and the protocatechuate decarboxylase has a catalytic activity of producing methacrylic acid from mesaconic acid or an isomer thereof.
A protocatechuate decarboxylase in which an amino acid at position 327 of an amino acid sequence represented by SEQ ID NO:
- 2 or an amino acid corresponding to the site is modified to methionine, phenylalanine, asparagine, isoleucine, glutamine, serine, valine, threonine, tyrosine, or leucine, and at least one amino acid modification described in the following (a) to (e) is performed:
- (a) modifying an amino acid at position 185 of the amino acid sequence represented by SEQ ID NO:
- 2 or an amino acid corresponding to the site to valine, isoleucine, methionine, threonine, serine, asparagine, leucine, tyrosine, histidine, or glutamine;
- (b) modifying an amino acid at position 331 of the amino acid sequence represented by SEQ ID NO:
- 2 or an amino acid corresponding to the site to valine or isoleucine;
- (c) modifying an amino acid at position 298 of the amino acid sequence represented by SEQ ID NO:
- 2 or an amino acid corresponding to the site to valine, glutamine, isoleucine, alanine, or leucine;
- (d) modifying an amino acid at position 183 of the amino acid sequence represented by SEQ ID NO:
- 2 or an amino acid corresponding to the site to leucine, tyrosine, glutamic acid, or methionine;
- (e) modifying an amino acid at position 438 of the amino acid sequence represented by SEQ ID NO:
- 2 or an amino acid corresponding to the site to isoleucine, methionine, valine, or threonine.
A method for producing protocatechuate decarboxylase having enhanced catalytic activity of producing methacrylic acid from mesaconic acid or an isomer thereof, the method comprising:
- modifying an amino acid at position 327 of an amino acid sequence represented by SEQ ID NO:
- 2 or an amino acid corresponding to the site to methionine, phenylalanine, asparagine, isoleucine, glutamine, serine, valine, threonine, tyrosine, or leucine in protocatechuate decarboxylase.
An agent for decarboxylating mesaconic acid or an isomer thereof and promoting production of methacrylic acid, comprising:
- protocatechuate decarboxylase, DNA encoding the protocatechuate decarboxylase, or a vector into which the DNA is inserted.
Dependencies are inferred from the claim text.