Improving enzymatic channeling efficiency via mixed nanoparticle scaffolds within self-assembled nanoparticle enzyme clusters

An enzymatic cluster composition comprising:

• a cluster of nanoparticles having a plurality of enzymes bound thereto via metal affinity coordination between histidine tags on the enzymes and zinc-containing surfaces of the nanoparticles,
• wherein the plurality of enzymes are configured as an enzymatic cascade,
• wherein the cluster of nanoparticles comprises both quantum dots and nanoplatelets,
• wherein the quantum dots comprise CdSe/CdS/ZnS core/shell/shell quantum dots, and
• wherein the nanoplatelets comprise CdSe/ZnS core/shell nanoplatelets.

A method of enhancing enzymatic cascade reactions, the method comprising:

• providing quantum dots comprising CdSe/CdS/ZnS core/shell/shell quantum dots;
• providing nanoplatelets comprising CdSe/ZnS core/shell nanoplatelets;
• providing a plurality of enzymes configured as an enzymatic cascade, wherein the enzymes comprise histidine tags;
• and
• combining the quantum dots, nanoplatelets, and enzymes under conditions that promote metal affinity coordination between the histidine tags and zinc-containing surfaces of the nanoparticles to form clustered nanoparticle-enzyme assemblies.