Methods and compositions for protein sequencing
AssigneeQuantum-Si Incorporated
InventorsBrian Reed, Manjula Pandey
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Abstract
Aspects of the application provide methods of identifying and sequencing proteins, polypeptides, and amino acids, and compositions useful for the same. In some aspects, the application provides amino acid recognition molecules, such as amino acid binding proteins and fusion polypeptides thereof. In some aspects, the application provides amino acid recognition molecules comprising a shielding element that enhances photostability in polypeptide sequencing reactions.
Claims21
1-151. (canceled)
An amino acid recognition molecule comprising:
- a first ClpS protein comprising a first amino acid sequence;
- a peptide linker comprising at least two amino acids;
- and
- a second ClpS protein comprising a second amino acid sequence,
- wherein the peptide linker covalently joins a terminal amino acid of the first amino acid sequence to a terminal amino acid of the second amino acid sequence, thereby forming a fusion polypeptide comprising the first and second ClpS proteins.
A nucleic acid encoding a fusion polypeptide, wherein the fusion polypeptide comprises:
- a first ClpS protein comprising a first amino acid sequence;
- a peptide linker comprising at least two amino acids;
- and
- a second ClpS protein comprising a second amino acid sequence,
- wherein the peptide linker covalently joins a terminal amino acid of the first amino acid sequence to a terminal amino acid of the second amino acid sequence.
Dependencies are inferred from the claim text.