Tissue and cell-type specific delivery of therapeutic molecules incorporating viral and human fusiogenic proteins

A non-immunogenic composition for delivery of a target molecule comprising:

• a first fusion peptide having a membrane-fusion moiety and a first component of a split complement system;
• a second fusion peptide having a second component of the split complement system and a first dimerization domain;
• a third fusion peptide having a second dimerization domain and a target molecule;
• wherein said membrane-fusion moiety is configured to be anchored to a cell membrane of a cell that forms an extracellular vesicle (EV);
• wherein the first, second, and third fusion peptides form a trimeric complex thereby loading said target molecule into said EV forming a gectosome for delivery to a target cell;
• and
• wherein said split component system is a split Vex-GFP system, and wherein said first dimerization domain and said second dimerization domains are nanobodies, and wherein said first dimerization domain is nanobody CA14, and said second dimerization domain is DB21.