Novel recombinant factor c and method for producing the same, and method for measuring endotoxin
AssigneeSeikagaku Corporation
InventorsHikaru MIZUMURA, Toshio ODA, Shun-ichiro KAWABATA
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Abstract
A horseshoe crab Factor C protein having activity of Factor C, wherein the horseshoe crab is selected from Tachypleus tridentatus, Limulus polyphemus, and Carcinoscorpius rotundicauda, and wherein the horseshoe crab Factor C protein is produced through being recombinantly expressed from a Chinese Hamster Ovary (CHO) DG44 cell or HEK cell.
Claims13
A human embryonic kidney (HEK) 293 cell comprising a polynucleotide that encodes a horseshoe crab Factor C protein having 90% or greater identity to SEQ ID NO:
- 2 and having enzymatic activity of Factor C, and wherein the horseshoe crab Factor C protein exhibits a residual activity of at least one selected from the group consisting of:
- (1) 10% or higher in the presence of 21 mM sodium citrate;
- and
- (2) 15% or higher in the presence of 16 mM magnesium sulfate.
A human embryonic kidney (HEK) 293 cell comprising a polynucleotide that encodes a horseshoe crab Factor C protein having 90% or greater identity to SEQ ID NO:
- 2 comprising a polynucleotide encoding a horseshoe crab Factor C protein having 90% or greater identity to SEQ ID NO:
- 2, and wherein the horseshoe crab Factor C protein exhibits a higher residual activity in the presence of a salt as compared to a control horseshoe crab Factor C protein.
A human embryonic kidney (HEK) 293 cell comprising a polynucleotide encoding a horseshoe crab Factor C protein having 90% or greater identity to SEQ ID NO:
- 2, wherein the horseshoe crab Factor C protein exhibits a higher residual activity in the presence of a salt as compared to a control horseshoe crab Factor C protein, wherein the salt is selected from the group consisting of sodium citrate and magnesium sulfate, and wherein the control horseshoe crab Factor C protein is a native Factor C protein of Tachypleus tridentatus.
A human embryonic kidney (HEK) 293 cell comprising a polynucleotide encoding a horseshoe crab Factor C protein having 90% or greater identity to SEQ ID NO:
- 2, wherein the horseshoe crab Factor C protein exhibits a higher residual activity in the presence of a salt as compared to a control horseshoe crab Factor C protein, wherein the salt is selected from the group consisting of sodium citrate and magnesium sulfate, and wherein the control horseshoe crab Factor C protein is a Tachypleus tridentatus Factor C protein produced by recombinant expression from an Sf9 cell.
Dependencies are inferred from the claim text.