High-speed, high-resolution optical system for large-field-of-view optical system for live tissue functional imaging
AssigneesThe Board of Trustees of the Leland Stanford Junior University, Howard Hughes Medical Institute
InventorsRadoslaw Chrapkiewicz, Mark J. Schnitzer
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Abstract
The present invention provides a microscopy imaging system with high spatial and temporal resolution and large field of view. One embodiment provides a combination of large field-of-view (8 mm) with high numerical aperture (0.47), superior temporal resolution (up to 1000 Hz) while maintaining high spatial resolution (i.e., less than 6 μm), dual-channel synchronized imaging capability, enhanced light collection efficiency (˜85% transmission), and LED synchronization with rolling shutter mechanism of the sCMOS cameras.
Claims23
An imaging system for fluorescence microscopy, comprising:
- a) an optical illumination system with one or multiple light sources, configured to provide illumination across distinct spectral bands;
- b) an objective lens with a numerical aperture of at least 0.2, providing a minimum field of view of 5 mm and a lateral optical resolution of 15 micrometers or better, configured to transmit the illumination to a specimen plane and detect fluorescence signals;
- c) an optical image detection system with one or multiple cameras, each configured to capture images of the fluorescence signals at an acquisition speed of at least 5 Hz.
A system for synchronized multi-spectral imaging comprising:
- a) at least one CMOS camera operating with a rolling shutter mechanism;
- b) pulsed light sources emitting at different excitation wavelengths;
- c) control circuitry generating a synchronization signal based on a temporal overlap of an exposure of first and last camera sensor rows, wherein the synchronization signal is used to trigger the pulsed light sources;
- d) a module configured to sequentially activate the pulsed light sources based on pulse counts or predefined sequences;
- e) spectral filters configured to separate excitation and emission wavelengths;
- whereby the system enables multi-spectral imaging at a frame rate of at least 100 Hz for 2048 camera rows acquisition while eliminating spatiotemporal artifacts associated with rolling shutter operation.
Dependencies are inferred from the claim text.