Engineered recombinant protein-binding domains as detection reagents
AssigneeEpiCypher, Inc.
InventorsMartis W. Cowles, Zu-Wen Sun, Bryan Venters, Matthew Marunde, Jonathan Burg, Michael-Christopher Keogh
Life of this patent
Estimating the term…
Abstract
Disclosed herein are methods to assay and identify detection reagents for chromatin elements, including recombinant fusion protein detection reagents comprising two or more binding domains for chromatin elements. Improved genomic mapping assays using the identified recombinant proteins are also disclosed. Recombinant fusion proteins optimized by the disclosed methods are also provided.
Claims31
A method for screening a detection reagent for a chromatin element, comprising:
- a. providing a panel of recombinant nucleosomes, each nucleosome comprising one or more chromatin elements recognized by one or more binding domains present in the detection reagent and/or one or more chromatin elements not recognized by the one or more binding domains present in the detection reagent, thereby providing both on-target and off-target recombinant nucleosomes;
- and
- b. performing a genomics assay or binding assay with the panel of recombinant nucleosomes to identify the binding specificity and/or efficiency of the detection reagent.
(canceled)
7-8. (canceled)
10-12. (canceled)
14-15. (canceled)
(canceled)
(canceled)
22-23. (canceled)
25-26. (canceled)
A recombinant fusion protein comprising two or more chromatin element binding domains, a label, and optionally a linker.
31-41. (canceled)
(canceled)
46-53. (canceled)
Dependencies are inferred from the claim text.