Single cell proteomics using degradable hydrogels
AssigneeCellanome, Inc.
InventorsPier Federico GHERARDINI, Tarun Kumar KHURANA, Stephen C. Macevicz
Life of this patent
Estimating the term…
Abstract
The present disclosure is directed to methods for measuring proteomes of single cells. In one aspect, methods comprise disposing cells on a surface, synthesizing hydrogel chambers around each of the cells, lysing the cells to release intracellular proteins for adsorption onto the surface bounded by the hydrogel chambers, depolymerizing the hydrogel chambers, and quantifying the adsorbed proteins with detection antibodies to determine the single cell proteomes.
Claims30
A method of determining proteomes of single cells, comprising:
- (a) synthesizing one or more gel chambers enclosing each of one or more cells disposed on or adjacent to a surface of a channel;
- (b) lysing the one or more cells so that cellular proteins of the one or more cells are released into the one or more gel chambers and at least a portion of the cellular proteins are adsorbed onto the surface enclosed by the one or more gel chambers;
- and
- (c) detecting the adsorbed cellular proteins with detection antibodies.
A method of determining proteomes of single cells comprising:
- (a) synthesizing one or more gel chambers enclosing each of the one or more cells disposed on a surface of a channel;
- (b) fixing and permeabilizing the one or more cells so that intracellular proteins of each cell are accessible to detection antibodies;
- and
- (c) detecting the intracellular proteins with the detection antibodies.
Dependencies are inferred from the claim text.