Method for Cellular Lifespan Measurement

A method for high-throughput screening to determine cellular lifespan, the method comprising the steps of:

• a) treating, in a well of a first multi-well plate, a sample containing cells with a condition that modulates lifespan of cells;
• b) transferring a portion of the cells in the well of the first multi-well plate into a well of a second multi-well plate;
• c) mixing, in a well of the second multi-well plate, the portion of cells with a fluorescent viability dye to stain the cells;
• d) measuring fluorescence intensity of the stained cells in a plate reader to obtain a fluorescence intensity value;
• e) measuring optical density of the cells in the plate reader to obtain an optical density value;
• and
• f) normalizing the fluorescence intensity value with the optical density value, wherein the normalized fluorescence intensity value directly correlates with the viability of the cells.

A method for high-throughput screening to determine cellular lifespan, the method comprising the steps of:

• a) plating, in a well of the multi-well plate, a sample containing cells and a fluorescent viability dye to stain the cells,
• b) treating, in the well of the multi-well plate, the sample containing cells with a condition that modulates lifespan of cells;
• and
• c) measuring fluorescence intensity of the stained cells in a plate reader to obtain a fluorescence intensity value.