Systems and Methods for Multiphoton Microscopy

Abstract

Multiphoton microscopy provides a non-invasive tool capable of monitoring metabolic states and/or the overall health of live cells with improved spatial resolution. A laser light source (e.g., a femtosecond laser) is used to excite one or more fluorophores, harmonophores, or other molecules in a biological sample and photonics are used to image, monitor, and retain cell health. Phototoxicity is reduced by rapidly scanning a laser light source over the sample such that full triplet relaxation in the sample is obtained, thereby reducing phototoxicity.

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