Enzymatic Modification of Saponins
AssigneeGlaxosmithkline Biologicals SA
InventorsMurray BROWN, Edward CHAPMAN, Andrew John COLLIS, Douglas E. FUERST, Joseph HOSFORD, Christopher M. MACDERMAID, Grégory MATHY, James Patrick MORRISON
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Abstract
Methods for the enzymatic modification of saponins produced in plant cell culture, products made thereby, uses of said products and also to other associated aspects. The saponins may be obtained from plant cell culture extracts of Quillaja species, such as extracts of Quillaja saponaria Molina.
Claims31
A method for making a product saponin, the method comprising:
- (i) providing a plant cell culture extract comprising saponins;
- and
- (ii) enzymatically converting a starting saponin from the plant cell culture extract to the product saponin.
A method for making a product saponin, the method comprising:
- (i) culturing plant cells that are capable of synthesis of saponins under conditions leading to the synthesis of the saponins;
- (ii) recovering the saponins from the plant cell culture;
- and
- (iii) enzymatically converting a starting saponin from the recovered saponins to the product saponin.
A method for making a product saponin, the method comprising:
- (i) culturing plant cells that are capable of synthesis of saponins under conditions leading to the synthesis of the saponins;
- (ii) enzymatically converting a starting saponin from the synthesized saponins to the product saponin;
- and
- (iii) recovering saponins from the plant cell culture.
A method for increasing an amount of a product saponin obtainable from a plant cell culture, the method comprising:
- (i) culturing plant cells that are capable of synthesis of saponins under conditions leading to the synthesis of the saponins;
- (ii) recovering the saponins from the plant cell culture;
- and
- (iii) enzymatically converting a starting saponin from the recovered saponins to the product saponin.
A method for increasing an amount of a product saponin obtainable from a plant cell culture, the method comprising:
- (i) culturing plant cells that are capable of synthesis of saponins under conditions leading to the synthesis of the saponins;
- (ii) enzymatically converting a starting saponin from the synthesized saponins to the product saponin;
- and
- (iii) recovering saponins from the plant cell culture.
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A method for producing saponins by plant cell culture, the method comprising:
- (i) culturing plant cells that are capable of synthesis of the saponins under conditions leading to the synthesis of the saponins;
- and
- (ii) recovering saponins,
- wherein the yield of a product saponin is increased by enzymatically converting a starting saponin from the recovered saponins to the product saponin.
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Dependencies are inferred from the claim text.