Image standardization method and observation apparatus

Abstract

According to this image standardization method, first, a region corresponding to a whole or a part of a biological sample in an image is extracted as an observation target region. Subsequently, an aggregate value of intensity values is calculated for each of multiple observation target regions. After that, intensity values of the multiple observation target regions are adjusted so that a difference in the aggregate value becomes smaller. This reduces variation in brightness among the multiple observation target regions. This can reduce a difference in an intensity value of the observation target region for the image of the biological sample including multiple cells. Consequently, multiple biological samples can be fairly observed and evaluated.

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