Tandem adaptive optics and fascile multi-color advances to structured illumination microscopy

A structured illumination microscopy system comprising:

• a plurality of light sources, wherein each light source in the plurality of light sources emits an excitation beam, and wherein the excitation beams vary in wavelength;
• a plurality of dichroic mirrors positioned to collimate the excitation beams emitted from the plurality of light sources into a multicolor beam;
• a blazed grating positioned to receive the multicolor beam and to disperse the multicolor beam into a plurality of monochrome beams, wherein the blazed grating directs the plurality of monochrome beams toward a digital micromirror device;
• the digital micromirror device positioned to receive the plurality of monochrome beams from the blazed grating and to direct the plurality of monochrome beams toward a sample;
• and
• an image sensor positioned between the digital micromirror device and the sample, wherein the image sensor generates an image of the sample based at least in part on an interaction of the plurality of monochrome beams and the sample.