Multivalent linkers used for antibody labeling
AssigneeProteintech Group, Inc.
InventorsJianxun LI, Deepa Shankar, Michael Metterlein, Sabrina Wendler, Andrea Buchfellner, Christian Linke-Winnebeck, Larisa Yurlova
Life of this patent
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Abstract
The disclosure relates to the use of multivalent linkers to label targets (e.g., primary antibodies). The disclosure provides methods of labeling antibody with a reporter/dye/enzyme via the multivalent linker. The disclosure further provides methods to use the multivalent linkers to label multiple primary antibodies of the same species in the same experiment.
Claims133
A multivalent linker that specifically binds a target antigen unit, said multivalent linker comprising:
- a) a plurality of peptide binding arms, each binding arm capable of binding to an epitope in the same target antigen unit;
- and
- b) at least one linker segment covalently operably linked to the plurality of peptide binding arms.
A molecular complex, comprising:
- (i) a single target antigen unit;
- and
- (ii) a multivalent linker comprising:
- a) a plurality of peptide binding arms, wherein each peptide binding arm binds to the single target antigen unit;
- and
- b) at least one linker segment covalently operably linked to the plurality of peptide binding arms.
A molecular complex, comprising:
- (a) a single target antigen unit comprising:
- i) a constant or Fc region of an antibody, said constant or FC region being selected from:
- 1) a human IgG1, IgG2, IgG3, or IgG4;
- 2) a murine IgG1, IgG2a, IgG2b, IgG2c or IgG3;
- 3) a rat IgG1, IgG2a, IgG2b, or IgG2c;
- or
- 4) a rabbit IgG;
- and
- (b) a multivalent linker comprising:
- i) a plurality of peptide binding arms, wherein each peptide binding arm binds to the single target antigen unit, wherein the peptide binding arm is a VHH of a camelid heavy chain antibody;
- and
- ii) at least one peptide linker segment covalently operably linked to the plurality of peptide binding arms, wherein the peptide linker segment is between 10 and 40 amino acids long, and wherein at least 80%, at least 85%, at least 90%, at least 95%, or 100%, of the amino acids in the linker segment are selected from glycine (G), alanine (A), serine (S), threonine (T), glutamic acid (E), aspartic acid (D), lysine (K) and arginine (R).
Dependencies are inferred from the claim text.