Rapid, single-step and reagentless electrochemical biosensing methods, compositions and devices for nanomolar detection of c-reactive protein

Abstract

An electrochemical biosensing method using screen-printed gold electrodes functionalized with a redox probe modified DNA aptamer that binds specifically to CRP is provided. Binding-induced conformational switching of the CRP-targeting aptamer induces a specific and selective signal-ON event, which enables single-step and reagentless detection of CRP in as little as 1 minute. The aptasensor limit of detection spans approximately 20-60 nM in 50% human serum with dynamic response windows spanning 1-200 or 1-500 nM (R=0.97/R=0.98 respectively). The sensor also operates in undiluted human blood producing an LOD of 45 nM equating to 5.4 mg/L. The sensor is stable for at least 1 week and can be reused numerous times, as judged from repeated real-time dosing and dose-response assays.

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