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US 2025/0154610 A1Published application
Methods and compositions for detecting candida species
AssigneeGen-Probe Incorporated
InventorsAngela S. HUDSON, Damon K. GETMAN, Alice JIANG, Barbara EATON
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Abstract
Disclosed are methods utilizing specific amplification of Candida sp. target nucleic acid for detecting the presence or absence of Candida sp. in a sample. Also disclosed are corresponding oligomers, including amplification oligomers, capture probes and detection probes, and combinations thereof, as well as corresponding reaction mixtures and kits.
Claims93
A method for determining the presence or absence of Candida sp. In a sample, the method comprising:
- (1) contacting a sample, said sample suspected of containing Candida sp., with at least one of a first amplification oligomer combination and a second amplification oligomer combination, wherein
- (a) the first amplification oligomer combination comprises first and second Candida-specific amplification oligomers for amplifying a first Candida sp. Target nucleic acid region or a second Candida sp. Target nucleic acid region, wherein said first target region corresponds to a region of SEQ ID NO:129 from about nucleotide position 133 or 161 to about nucleotide position 259 and said second region corresponds to a region of SEQ ID NO:130 from about nucleotide position 202 to about nucleotide position 308, and wherein the first and second Candida-specific amplification oligomers respectively comprise first and second Candida-specific target-hybridizing sequences;
- and
- (b) the second amplification oligomer combination comprises first and second C. glabrata-specific amplification oligomers for amplifying a third Candida sp. Target nucleic acid region, wherein said third target region corresponds to a region of SEQ ID NO:131 from about nucleotide position 355 to about nucleotide position 554, and wherein the first and second C. glabrata-specific amplification oligomers respectively comprise first and second C. glabrata-specific target-hybridizing sequences;
- (2) performing an in vitro nucleic acid amplification reaction, wherein any Candida sp. Target nucleic acid, if present in the sample, is used as a template for generating one or more amplification products corresponding to at least one of the first, second, and third target regions;
- and
- (3) detecting the presence or absence of the one or more amplification products, thereby determining the presence or absence of Candida sp. In the sample.
4 more elements
An oligomer combination for determining the presence or absence of Candida sp. In a sample, the oligomer combination comprising:
- at least one of a first amplification oligomer combination and a second amplification oligomer combination, wherein
- (a) the first amplification oligomer combination comprises first and second Candida-specific amplification oligomers for amplifying a first Candida sp. Target nucleic acid region or a second Candida sp. Target nucleic acid region, wherein said first target region corresponds to a region of SEQ ID NO:129 from about nucleotide position 133 or 161 to about nucleotide position 259 and said second region corresponds to a region of SEQ ID NO:130 from about nucleotide position 202 to about nucleotide position 308, and wherein the first and second Candida-specific amplification oligomers respectively comprise first and second Candida-specific target-hybridizing sequences;
- and
- (b) the second amplification oligomer combination comprises first and second C. glabrata-specific amplification oligomers for amplifying a third Candida sp. Target nucleic acid region, wherein said third target region corresponds to a region of SEQ ID NO:131 from about nucleotide position 355 to about nucleotide position 554, and wherein the first and second C. glabrata-specific amplification oligomers respectively comprise first and second C. glabrata-specific target-hybridizing sequences.
1 more element
A detection probe for detecting a Candida sp. Target nucleic acid, wherein said detection probe is
- a Candida-specific detection probe comprising a Candida-specific detection probe target-hybridizing sequence selected from the group consisting of
- (A1) a sequence of from 18 to 22 contiguous nucleotides contained in the sequence of SEQ ID NO:145 and that includes at least the sequence of SEQ ID NO:146,
- (B1) the DNA equivalent or an RNA/DNA chimeric of (A1), and
- (C1) the full complement of (A1) or (B1), or
- a C. glabrata-specific detection probe comprising a C. glabrata-specific detection probe target-hybridizing sequence selected from the group consisting of
- (A2) a sequence of from 17 to 23 contiguous nucleotides contained in the sequence of SEQ ID NO:147 and that includes at least the sequence of SEQ ID NO:148,
- (B2) a sequence that substantially corresponds to the sequence of residues 1-17 of SEQ ID NO:18,
- (C2) a sequence that substantially corresponds to the sequence of residues 1-20 of SEQ ID NO:21,
- (D2) the DNA equivalent or an RNA/DNA chimeric of any of (A2)-(C2), and
- (E2) the full complement of any one of (A2)-(D2).
7 more elements
Dependencies are inferred from the claim text.