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US 2025/0154601 A1Published application
Method for hormone or drug screening in a tissue sample
AssigneeEcole Polytechnique Federale de Lausanne
InventorsYueyun ZHANG, Christoph MERTEN, Cathrin BRISKEN
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Abstract
The present invention relates to a method for analyzing cellular responsiveness to hormones or drags in a tissue sample based on an alginate-based 3D ex vivo culture system for normal and malignant tissue microstructures and uses thereof.
Claims15
A method for analyzing cellular responsiveness to hormones or drugs in a tissue sample from a patient or in a patient-derived tissue sample, comprising the steps of:
- a) Mechanical and/or enzymatic digestion of said tissue sample to produce tissue microstructures;
- b) Encapsulation of said tissue microstructures in alginate;
- c) Exposing the encapsulated tissue microstructure to hormones or drugs;
- d) Cell lysis, mRNA capture and reverse transcription in one-step;
- e) Alginate dissociation;
- f) RNA sequencing;
- wherein step d) is performed with the alginate still crosslinked and intact.
4 more elements
Dependencies are inferred from the claim text.