Non-coding rna for detection of cancer
AssigneeThe Regents of the University of California
InventorHani Goodarzi
Life of this patent
Estimating the term…
Abstract
The present disclosure relates generally to detection on non-coding RNA molecules in a sample or diagnosis of subject based upon detection or quantification of non-coding nucleic acid sequences in a sample, specifically to identify and use of molecular biomarkers for cancer including breast cancer.
Claims21
A method of diagnosing a subject with a benign, pre-malignant, or malignant hyperproliferative cell comprising:
- detecting the presence, absence, and/or quantity of at least one orphan non-coding RNA (oncRNA) or functional fragment thereof in a sample from the subject using one or more probe specific for oncRNA.
A method of detecting a cancer cell in a subject comprising:
- detecting whether a oncRNA is present in a sample of the subject by contacting the sample with an amount of one or a combination of probes complementary to one or a combination of oncRNA sequences.
A method of treating a subject in need thereof diagnosed with or suspected of having breast cancer, comprising:
- (a) contacting one or a plurality of probes specific for one or a combination of oncRNAs and/or a homologous sequence thereof with a sample;
- (b) quantifying the presence, absence or amount of oncRNA and/or a homologous sequence thereof in the sample;
- (c) calculating one or more scores based upon the presence, absence, or quantity of oncRNA and/or a homologous sequence thereof;
- (d) correlating the one or more scores to the presence, absence, or quantity of oncRNA and/or a homologous sequence thereof, such that, if the amount of oncRNA and/or a homologous sequence thereof is greater than the quantity of oncRNA and/or a homologous sequence thereof in a control sample, the correlating step comprises diagnosing a subject with breast cancer;
- and
- (e) administering to the subject a therapeutically effective amount of treatment for the breast cancer.
A method of diagnosing a subject with a benign, pre-malignant, or malignant hyperproliferative cell comprising:
- detecting the presence, absence, and/or quantity of one or more oncRNA or functional fragment thereof in a sample from the subject using one or more probe specific for an oncRNA selected from SEQ ID NO:3, SEQ ID NO:
- 19, SEQ ID NO:32, SEQ ID NO:
- 40, SEQ ID NO:41, SEQ ID NO:
- 79, SEQ ID NO:82, SEQ ID NO:83, SEQ ID NO:
- 126, SEQ ID NO:
- 148 and SEQ ID NO:
- 191.
Dependencies are inferred from the claim text.