Rapid next-generation gene sequencing method

Abstract

A rapid next-generation gene sequencing method includes the following steps: incorporation: combining a detection complex to a primer hybridized to a nucleic acid molecule to be detected, wherein the detection complex is modified with a fluorescence group and a blocker group; scanning: exciting the fluorescence group to generate a fluorescence signal, obtaining an image signal through an optical imaging system, and identifying the image signal to obtain the type of a base; cleavage: removing the fluorescence group and the blocker group; repeating the cycle of the incorporation-scanning-cleavage. The scanning is started after the beginning of the incorporation step, and/or the cleavage is started after the beginning of the scanning step. In the sequencing method, unfinished incorporation reaction and preparatory work for the cleavage reaction are performed during the signal scanning period, which reduces the time of a single cycle, thereby finally shortening the time for the entire sequence process.

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