Systems and methods for sequencing error correction via double strand preservation

A method for sequencing error correction for a double-stranded nucleic acid molecule, comprising:

• a. contacting a support comprising a plurality of identical surface primers with a template comprising a first strand and a second strand hybridized to the first strand, wherein the template comprises a locus with a base mismatch error;
• b. amplifying the template to generate an amplified support comprising a plurality of nucleic acid molecules attached thereto, wherein each molecule of a first subset of the plurality of nucleic acid molecules comprises a copy of at least a portion of the first strand and each molecule of a second subset of the plurality of nucleic acid molecules comprises a copy of at least a portion of a reverse complement copy of the second strand;
• c. sequencing the plurality of nucleic acid molecules by collecting a plurality of sequencing signals from the amplified support;
• and
• d. generating a sequencing read for the template from the plurality of sequencing signals, wherein the sequencing read does not include a base call at the locus with the base mismatch error based on identifying a phasing event from the plurality of sequencing signals at or downstream of the locus.