Inward fluid displacement (ifd) in rotational microfluidic device

A method for performing processing of sample using a rotationally-driven microfluidic assembly, the method comprising:

• conveying a sample to a sample chamber of the microfluidic assembly from a sample inlet region by rotating the microfluidic assembly about a center of rotation (CoR) defined by a hub region;
• actuating a liquid reagent valve to fluidically connect a liquid reagent chamber with a dry reagent region of the microfluidic assembly;
• conveying a liquid reagent from the liquid reagent chamber to the dry reagent region by rotating the microfluidic assembly about the CoR;
• isolating the liquid reagent chamber from the dry reagent region by re-sealing the liquid reagent valve;
• and
• propelling the sample to a sample recovery chamber from the sample chamber using a gas evolved from a reaction between the liquid reagent and a dry reagent located in the dry reagent region, the gas conveyed to the sample chamber using a gas transfer channel from the dry reagent region;
• wherein the sample recovery chamber is located more proximally to the CoR as compared to the dry reagent region and the sample chamber;
• and
• wherein the sample is propelled at least in part inwardly toward the CoR by the gas.