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US 2025/0154558 A1Published application
Methods and systems for analyzing nucleic acid molecules
AssigneeGuardant Health, Inc.
InventorAndrew KENNEDY
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Abstract
The disclosure provides methods for processing nucleic acid populations containing different forms (e.g., RNA and DNA, single-stranded or double-stranded) and/or extents of modification (e.g., cytosine methylation, association with proteins). These methods accommodate multiple forms and/or modifications of nucleic acid in a sample, such that sequence information can be obtained for multiple forms. The methods also preserve the identity of multiple forms or modified states through processing and analysis, such that analysis of sequence can be combined with epigenetic analysis.
Claims20
A method comprising:
- a) providing one or more populations of cell-free DNA from a biological sample from a subject;
- b) fractionating the one or more populations of cell-free DNA based on characteristics that are present at different levels in cell-free DNA derived from cancerous compared to noncancerous cells, thereby generating subpopulations of cell-free DNA, wherein the characteristics are:
- (i) methylation level of the cell-free DNA;
- and
- (ii) protein modifications of histones;
- c) amplifying at least one of the subpopulations of cell-free DNA;
- and
- d) sequencing at least one of the amplified subpopulations of cell-free DNA.
5 more elements
Dependencies are inferred from the claim text.