Single-molecule rna structure profiling

Abstract

Described is a method for determining structure of an RNA molecule, the method comprising: a) subjecting a population of RNA molecules to structure-specific chemical modifications such that individual RNA molecules are modified; b) reverse-transcribing the modified RNA to provide a complementary DNA (cDNA) molecule, and generating double stranded DNA from said cDNA molecule; c) performing single-molecule sequencing of the double stranded cDNA using a sequencing format which provides multiple reads of each molecule to arrive at a consensus sequence representing a chemical mutation profile for an individual DNA; d) using said chemical mutation profile to determine likelihood of an RNA molecule being single stranded or double stranded at each individual base, to thereby determine the structure of the RNA molecule.

Dependencies are inferred from the claim text.