Glycosyltransferase variant, and method of preparing steviol glycosides using same
AssigneeSamyang Corporation
InventorsJeongmin KIM, Hyeokjin KO, Junho MOON, Eunsoo CHOI
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Abstract
The present invention relates to a glycosyltransferase mutant, and a composition and a method for producing steviol glycosides using the same, and more specifically, to a glycosyltransferase of rebaudioside that transfers glucose to steviol glycosides and its mutant, a recombinant strain expressing the enzymes, and a method for producing rebaudioside using the same.
Claims17
An enzyme protein having a uridine diphosphate-glucosyltransferase (UDP-glucoslyl transferase) activity to transfer glucose to steviol glycoside substrate, wherein at least one amino acid selected from the group consisting of the 201st and 202nd amino acids from the N-terminus in the amino acid sequence of SEQ ID NO:
- 1 is substituted with at least one amino acid selected from the group consisting of serine and leucine.
Dependencies are inferred from the claim text.