Recombinant microorganism for producing threonine and use thereof
OwnerPei KANG, Tao WU +3inventors
InventorsPei KANG, Tao WU, Weibo GONG, Jun HE, Yan LI
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OwnerPei KANG, Tao WU +3inventors
InventorsPei KANG, Tao WU, Weibo GONG, Jun HE, Yan LI
Estimating the term…
Provided are a recombinant microorganism for producing threonine and the use thereof in the fermentation production of threonine or a derivative thereof. A 20-30 bp segment upstream of a start codon of a gene encoding phosphoenolpyruvate carboxylase in the recombinant microorganism is replaced with a strong promoter. By means of the specific optimization of the promoter of the gene encoding phosphoenolpyruvate carboxylase and the mutation of the encoding region of the gene, the ability of the strain to synthesize threonine is significantly improved.
A method for increasing the production of threonine production by a Corynebacterium species or in constructing a Corynebacterium species that produces threonine, the method comprising:
A recombinant microorganism, wherein, the 20-30 bp segment upstream of the start codon of a gene encoding phosphoenolpyruvate carboxylase in the recombinant microorganism is replaced with a strong promoter.
(canceled)
A method for fermentative production of threonine or a derivative thereof, comprising a step of culturing a recombinant microorganism, and isolating threonine or the derivative thereof from the culture,
Dependencies are inferred from the claim text.