Disruption of cdc42 effectors in yeast for increased alcohol and lysine production
AssigneeDanisco US Inc.
InventorsJaclyn Diana DeMartini, Celia Emily Gaby Payen
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Abstract
Described are compositions and methods relating to yeast having a genetic mutation that results in decreased amounts of Cdc42 effector proteins, resulting in increased alcohol and lysine production. Such yeast is well-suited for use commercial alcohol production to increase yields and to increase the value of Such yeast is well-suited for use commercial alcohol production to increase yields and to increase the value of amino-acid-containing, fermentation-co-products.
Claims21
A method for producing a modified yeast cell comprising:
- introducing a genetic alteration into a parental yeast cell, which genetic alteration reduces or prevents the production of functional Cdc42 effector polypeptides compared to the parental cells, thereby producing modified yeast cells that produce during fermentation an increased amount of alcohol and/or lysine compared to the parental cells under equivalent fermentation.
A method for increasing the amount of lysine present in a post-fermentation product, comprising:
- (i) hydrolyzing a starch-containing feedstock with an α-amylase to produce a starch liquefact;
- (ii) saccharifying the starch liquefact with a glucoamylase to produce glucose;
- (iii) fermenting the glucose with modified yeast cells derived from parental yeast cells, the modified yeast cells comprising a genetic alteration that causes the production of decreased amounts of functional Cdc42 effector polypeptides compared to the parental cells;
- and
- (iv) recovering a post-fermentation by-product enriched for lysine compared to post-fermentation by-product recovered from an otherwise identical process using the parental yeast.
A method for increasing the amount of lysine present in a fermentation product, comprising:
- (i) fermenting glucose or another sugar with modified yeast cells derived from parental yeast cells, the modified yeast cells comprising a genetic alteration that causes the production of decreased amounts of functional Cdc42 effector polypeptides compared to the parental cells;
- and
- (ii) recovering fermentation product enriched for lysine compared to a fermentation product recovered from an otherwise identical process using the parental yeast.
Dependencies are inferred from the claim text.