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US 2025/0154523 A1Published application
CELL-SPECIFIC EXPRESSION OF modRNA
AssigneeIcahn School of Medicine at Mount Sinai
InventorsLior ZANGI, Ajit MAGADUM
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Abstract
Provided is an expression regulatory system for expression of a gene of interest, including a first polynucleotide encoding a translation-suppressor protein and one or both of a miR1 and miR208 recognition element and a second RNA molecule encoding the gene of interest and including a recognition sequence for the translation-suppressor.
Claims20
An expression regulatory system for expression of a gene of interest, comprising
- a recombinant first RNA molecule, comprising (i) a coding sequence for a translation-suppressor protein and (ii) a microRNA (miR) recognition element in its 3′ UTR, wherein the first miR recognition element recognizes one or more miR and binding of one or more of the one or more miR to the miR recognition element reduces translation of the translation suppressor, and
- a recombinant second RNA molecule, comprising (i) a coding sequence for the gene of interest and (ii) a recognition sequence for the translation-suppressor, wherein binding of the translation-suppressor to the recognition sequence for the translation-suppressor reduces translation of the gene of interest, wherein
- the one or more miR recognition element is selected from a miR1 recognition element, a miR208 recognition element, and both a miR1 recognition element and a miR208 recognition element.
Dependencies are inferred from the claim text.