Method for vector insertion site detection and clonal quantification using tagmentation
AssigneesSeoul National University R&Db Foundation, Seoul National University Hospital
InventorsJong-il Kim, Jaeryuk Kim, Hyoung Jin Kang, Miyoung Park
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Abstract
The present disclosure relates to a method of detecting an integration site of a vector in a genome. According to the method of the present disclosure, it is possible to simply and quickly analyze a quantitative integration site of a viral vector with respect to a plurality of DNA motifs (sites) in a genome. Therefore, it can be useful for monitoring the safety and effectiveness of a gene therapy agent.
Claims13
A method of quantitatively detecting an integration site of a vector in a genome, comprising the following processes:
- a process of tagmentation using a bead-linked transposome;
- a process of preparing a library through gene amplification;
- a process of library pooling and sequencing;
- and
- a process of determining an integration site in a genome through bioinformatics analysis,
- wherein the process of preparing a library is performed by a 1st round polymerase chain reaction (PCR) and a 2nd round PCR (nested-PCR).
A method of quantifying clones with vectors integrated into genomes, comprising the following processes:
- a process of tagmentation using a bead-linked transposome;
- a process of preparing a library through gene amplification;
- a process of library pooling and sequencing;
- and
- a process of determining an integration site in a genome through bioinformatics analysis,
- wherein the process of preparing a library is performed by a 1st round PCR and a 2nd round PCR (nested-PCR).
Dependencies are inferred from the claim text.