Method for isolating nucleic acids by a novel hemp matrix
AssigneeTechnoculture Research Private Limited
InventorsAfreen Khanam, Sana Ul Fatma, . SATYAM
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Abstract
The present invention provides a method for nucleic acid extraction and purification from a biological sample by an alkali-cottonized hemp matrix that may be scaled up for industrial production of a novel alkali-cottonized hemp matrix. The methods/matrix as disclosed in the present invention is economically effective over the existing methods and uses the largely available hemp fiber for the process. The extraction achieved by the novel matrix of the present invention achieved better nucleic acid extraction over the existing methods/matrices.
Claims16
A method for isolation of pure nucleic acid from a biological sample, method comprising steps—
- a. loading the lysate sample to a column containing a matrix;
- and
- b. washing and eluting the sample-bound matrix,
- wherein the matrix is an alkali-cottonized hemp matrix.
An industrial system of extraction and purification of biological macromolecules from a sample, comprising:
- a. Autoclavable reagent storage tanks [104] configured to collect lysed/raw samples, wherein the said incubator bio-mixer [104] is temperature-, humidity-, and pressure-controlled;
- b. Incubator bio-mixer [106] configured to receive sample and lysate reagent via pressure pumps and regulators (P1, P2, P3, P4, . . . ) at a controlled flow rate from the individual, autoclavable reagent storage tanks [104];
- c. Matrix column chamber [110] comprising HEMP matrix column [102] connected to a Processing reagent chambers [112] comprising alkalization chambers configured to treat the cottonized hemp fiber with 5-50% alkali solution in deionized water followed by drying the hemp fibers for 30 min-48 hrs at 20-70° C., via pumps (P6, P7, P8, P9, P10, . . . Pn);
- d. Felting and Shredding Units configured to separate clogged cottonized hemp fibers into separate fibrous structures and interlocking of cottonized hemp fibers respectively.
- e. Automated mixer chamber [114] placed downstream of the matrix column chamber [110] configured to incubate and processing of samples, wherein the automated mixer chamber [114] is connected with reagent chambers via pumping (P12, P13, . . . Pn) for sample purification and extraction process;
- f. Optionally a solid-state purification column [116] for ultra purification;
- and
- g. Optionally Closed inert vials or sterilized sample containers [118] for storage of the processed samples.
Dependencies are inferred from the claim text.