Novel crispr dna and rna targeting enzymes and systems
AssigneeArbor Biotechnologies, Inc.
InventorsElise Keston-Smith, David A. Scott, David R. Cheng, Winston X. Yan, Pratyusha Hunnewell, Jason Carte
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Abstract
The disclosure describes novel systems, methods, and compositions for the manipulation of nucleic acids in a targeted fashion. The disclosure describes non-naturally occurring, engineered CRISPR systems, components, and methods for targeted modification of nucleic acids such as DNA. Each system includes one or more protein components and one or more nucleic acid components that together target nucleic acids.
Claims18
An engineered, non-naturally occurring Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR)-associated (Cas) system comprising:
- an RNA guide or a nucleic acid encoding the RNA guide, wherein the RNA guide comprises a direct repeat sequence, a spacer sequence capable of hybridizing to a target nucleic acid in a eukaryotic cell, and a tracrRNA;
- and
- a RuvC nuclease domain-containing CRISPR-Cas effector protein or a nucleic acid encoding the RuvC nuclease domain-containing CRISPR-Cas effector protein, wherein the RuvC nuclease domain-containing CRISPR-Cas effector protein is capable of binding to the RNA guide and of targeting the target nucleic acid complementary to the spacer sequence;
- wherein the RuvC nuclease domain-containing CRISPR-Cas effector protein comprises an amino acid sequence that is at least 80% identical to an amino acid sequence of any of SEQ ID NOs:
- 1-5, 7, 8, or 15-24.
Dependencies are inferred from the claim text.