ISPETASE-8xCHIMERA RECOMBINASE, ENCODING GENE, RECOMBINANT PLASMID, ENGINEERED STRAIN AND APPLICATIONS

An IsPETase-8×Chimera recombinase, comprising an amino acid sequence modified from an amino acid sequence of a wild-type polyethylene terephthalate (PET) hydrolase by replacing at least one of the amino acid sequences set forth in SEQ ID NOs:

• 1 to 8, wherein the amino acid sequence of the wild-type PET hydrolase comprises the amino acid sequences set forth in SEQ ID NOs:
• 1 to 8;
• wherein
• the amino acid sequence set forth in SEQ ID NO:
• 1 is replaced by the amino acid sequence set forth in SEQ ID NO:
• 9 or SEQ ID NO:
• 10;
• the amino acid sequence set forth in SEQ ID NO:
• 2 is replaced by the amino acid sequence set forth in SEQ ID NO:
• 11 or SEQ ID NO:
• 12;
• the amino acid sequence set forth in SEQ ID NO:
• 3 is replaced by the amino acid sequence set forth in SEQ ID NO:
• 13 or SEQ ID NO:
• 14;
• the amino acid sequence set forth in SEQ ID NO:
• 4 is replaced by the amino acid sequence set forth in SEQ ID NO:
• 15;
• the amino acid sequence set forth in SEQ ID NO:
• 5 is replaced by the amino acid sequence set forth in SEQ ID NO:
• 16 or SEQ ID NO:
• 17;
• the amino acid sequence set forth in SEQ ID NO:
• 6 is replaced by the amino acid sequence set forth in SEQ ID NO:
• 18;
• the amino acid sequence set forth in SEQ ID NO:
• 7 is replaced by the amino acid sequence set forth in SEQ ID NO:
• 19;
• and
• the amino acid sequence set forth in SEQ ID NO:
• 8 is replaced by the amino acid sequence set forth in SEQ ID NO:
• 20.