Creation of vascularized biological structures

Abstract

Compositions and methods are provided relating to in vitro differentiation of vascularized two-and three-dimensional biological structures, which may be referred to herein as vascularized organoids (VOs). Vascularization in the organoids is spatially organized, and can include hierarchical branching of large vessels to small vessels. Features of the vascularized organoids include vascular tissues having the same branching structure and self-organization as seen in vivo; and physiologically relevant spatial organization within endocardial, myocardial, epicardial, and/or progenitor cells. The organoids also have inherent vascular beds that can be anastomosed immediately with host vasculature beds, thereby improving transplantation efficiency by rapid oxygenation from blood flowing through their pre-incorporated vascular beds.

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