Novel dual culture method for culturing immune cells and natural killer cells, and use thereof

Abstract

Provided is a method for the dual culture of immune cells used for immunotherapy, and more specifically, to a dual culture method in which immune cells, e.g., immune cells (PBMCs), natural killer cells, and gamma delta T cells, which are highly effective in treating malignant tumors and the like, are selectively and efficiently amplified and activated by culturing lymphocytes derived from human peripheral blood, wherein the culture efficiency and activity can be increased when immune cells are cultured in combination after applying different stimuli for a predetermined period of time.

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