Compositions and methods for expanding immune cells
AssigneeRutgers, The State University of New Jersey
InventorsDongfang Liu, Xuening Wang
Life of this patent
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Abstract
Methods of expanding a population of immune cells, such as natural killer cells or CAR-modified natural killer cells, using exosomes, and methods of producing the exosomes from a population of 721.221 cells transduced or transfected with a membrane-bound IL-21 (mIL-21) or a population of 721.22 cells transduced or transfected with a mIL-21 and B7-H6. Methods of treating a cancer or an infectious or immune disease, wherein immune cells expanded using the exosomes are administered to a subject with the cancer or the infectious or immune disease are also provided.
Claims45
A method of expanding a population of immune cells, comprising:
- contacting the population of immune cells with an exosome isolated from a population of 721.221 cells transduced or transfected with a nucleic acid encoding membrane-bound IL-21 (mIL-21), wherein the contacting occurs under conditions sufficient for cell expansion;
- thereby expanding the population of immune cells.
A method of producing an isolated exosome, comprising:
- isolating the exosome from a supernatant of a culture of a population of 721.221 cells transduced or transfected with a nucleic acid encoding mIL-21;
- thereby producing the isolated exosome.
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A modified 721.221 cell expressing membrane-bound IL-21 (mIL-21) and B7-H6.
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