Method for Generating Regulatory T Cells
AssigneeOsaka University
InventorsShimon Sakaguchi, Norihisa Mikami
Life of this patent
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Abstract
Provided is a method for producing regulatory T cells, comprising the step of culturing mammalian-derived peripheral T cells in a medium comprising TGFβ and IL-2 in the presence of CD3 stimulation and in the absence of CD28 stimulation. The medium may further comprise ascorbic acid. The method may further comprise the step of culturing the obtained cell culture in a medium comprising IL-2.
Claims16
1-8 (canceled)
An immunological composition comprising an anti-CD23 monoclonal antibody and an anti-CD28 monoclonal antibody wherein at least one monoclonal antibody is immobilized on a surface of an insoluble carrier.
A flow cytometry method for isolating murine cells comprising the steps of (i) contacting isolated murine cells with an anti-CD4 murine monoclonal antibody, an anti-CD25 murine monoclonal antibody and an anti-CD45RA murine monoclonal antibody;
- (ii) sorting murine cells which are bound to anti-CD4 monoclonal antibody and anti-CD45RA monoclonal antibody and not bound to anti-CD25 monoclonal antibody from murine cells which are bound to anti-CD25 monoclonal antibody;
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- (iii) isolating the sorted murine cells which are bound to anti-CD4 murine monoclonal antibody and anti-CD45RA murine monoclonal antibody and not bound to anti-CD25 murine monoclonal antibody.
Dependencies are inferred from the claim text.