Method and means for an isolation of membrane-bound proteins from a biological sample
AssigneeBASF Plant Science GmbH
InventorsMeghan K. Wyrick, Min Xia, Janna Slessareva
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AssigneeBASF Plant Science GmbH
InventorsMeghan K. Wyrick, Min Xia, Janna Slessareva
Estimating the term…
The present invention provides the method and means for an efficient isolation of membrane-bound proteins from biological samples, e.g. in samples from raw or processed plant material. The biological sample can be highly processed, e.g. by applying high temperature, pressure, or a chemical treatment and can be derived from seed matrices as well as other typical plant tissues for example seed, grain, leaf, root, or pollen. The invention comprises the provision of a novel extraction buffer (MEB) and its application in the method of the invention.
A method for the extraction of membrane-bound proteins from grain meal or plant tissue comprising:
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