A functionalised chromatography medium lacking surface extender

Abstract

A chromatography medium is provided, comprising a matrix of cellulose-based nanofibers, the nanofibers optionally being crosslinked to one another. A ligand coupled to the matrix without any intermediate extender group. Also provided is a method of preparing a functionalised chromatography medium. The method comprises: (i) providing a substrate comprising cellulose acetate; (ii) forming a fibrous matrix/membrane spun of nanofibers from the substrate; (iii) saponification of the nanofibers to form regenerated cellulose nanofibers; (iv) derivatisation of the regenerated cellulose nanofibers with a cross-linker, and (v) coupling of a ligand to the derivatised cellulose nanofibers, wherein the preparation of the functionalised chromatography medium does not comprise any surface extender. The chromatography medium is useful for separation of large analytes, such as viruses.

Dependencies are inferred from the claim text.