Dna adjuvant hydrogel-based peptide vaccine, and preparation method and use thereof

Abstract

A DNA adjuvant hydrogel-based peptide vaccine, and a preparation method and use thereof are provided, belonging to the technical field of biological products. The DNA adjuvant hydrogel-based peptide vaccine includes a peptide-carrier protein, a DNA hydrogel, and a free carrier protein; where the peptide-carrier protein is loaded in the DNA hydrogel or encapsulated in the DNA hydrogel by hybridization; the free carrier protein is encapsulated inside the DNA hydrogel; and the DNA hydrogel is self-assembled from a Y-shaped scaffold and a linker DNA. In the peptide vaccine, the free carrier protein is rapidly released to induce CD4+ T cell activation; while the peptide-carrier protein is slowly released, thereby achieving B cell sensitization while activating more CD4+ T cells. In this way, B cells are effectively activated to enhance an immune effect of the peptide vaccine.

Dependencies are inferred from the claim text.