Edible plant-derived nanoparticles for regulation of gut microbiota
AssigneeUniversity of Louisville Research Foundation, Inc.
InventorHuang-Ge Zhang
Life of this patent
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Abstract
Provided are methods for modulating gut microbiota in subjects. In some embodiments, the methods include administering to a subject an effective amount of a composition that includes a first edible plant-derived nanoparticle encapsulating an effective amount of RNA. Also provided are methods for preventing and/or treating gut dysbiosis, methods for modulating bacterial growth, methods for modulating inflammatory cytokines, methods for reducing migration of bacterial from the gut to gut-associated bloodstream, and compositions for use in the presently disclosed methods, including pharmaceutical compositions.
Claims27
1-19. (canceled)
A method for modulating growth of a bacterium in a subject's digestive system, the method comprising administering to the subject an exosome-like nanoparticle (ELN) and/or a plant-derived nanovector (NV) in an amount and by a route sufficient to modulate the growth of the Lactobacillus bacterium in the subject's digestive system.
A method for modulating an inflammatory cytokine in the gut of a subject, the method comprising administering to the subject an effective amount of an edible plant-derived nanoparticle encapsulating an effective amount of RNA and/or an RNA isolated therefrom, whereby an inflammatory cytokine in the gut of the subject is modulated.
(canceled)
A method for reducing migration of Lactobacillaceae from the gut to gut-associated bloodstream of a subject, the method comprising:
- a. contacting Lactobacillaceae with a plurality of ginger exosome-like nanoparticles (GELNs) and/or ginger-derived nanovectors (GNVs), and/or RNA derived therefrom, under conditions sufficient for the GELNs and/or RNA derived therefrom to be taken up by the Lactobacillaceae;
- and
- b. administering the Lactobaciilaceae in an amount sufficient for the Lactobacillaceae to colonize the gut of the subject, whereby migration of the Lactobacillaceae from the gut to the gut-associated bloodstream of the subject is reduced.
(canceled)
A composition comprising a first edible plant-derived exosome-like nanoparticle (ELN) and/or a plant-derived nanovector (NV) encapsulating an effective amount of an RNA, the RNA obtained from a second edible-plant derived nanoparticle or nano vector.
The composition of claim 1, wherein the first edible plant-derived ELN and/or NV, the second edible plant-derived ELN and/or NV, or both are derived from an edible plant selected from the group consisting of ginger, grapefruit, carrot, garlic, and turmeric.
38-39. (canceled)
41-42. (canceled)
Dependencies are inferred from the claim text.