Cells having improved proliferative capacity and reduced cellular senescence
AssigneeBoard of Regents, The University of Texas System
InventorsAaron BAKER, Miles MASSIDDA
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Abstract
Provided herein are methods for mechanically conditioning cells to improve their proliferative function and multipotency, as well as protect them from advancing senescence. In the methods, brachial loading alone is first used to improve the efficiency of culture expansion. Once a sufficient population of healthy cells are generated, pharmacological agents are added as a cotreatment to drive differentiation into a desired phenotype. The conditioned cells may then be used to prepare compositions for administration to a patient to treat a disease.
Claims27
A method of preparing a therapeutic cell population, the method comprising:
- (a) adhering a starting population of cells on a flexible or expandable surface;
- (b) applying a brachial waveform of mechanical stretch to the starting population of cells, thereby generating a second population of cells;
- (c) expanding the second population of cells under static conditions;
- and
- (d) applying a brachial waveform of mechanical stretch to the expanded second population of cells in the presence of at least one pharmacological agent that induces differentiation of the cells, thereby generating the therapeutic population of cells.
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Dependencies are inferred from the claim text.