Methods for the production of tcr gamma delta + t cells

A method for expanding Vδ2−TCRγδ+ T cells in a sample comprising:

• culturing cells in the sample in a culture medium comprising a T cell mitogen which is an antibody or fragment thereof and interleukin-4;
• in the absence of a interleukin-15 and in the absence of feeder cells;
• or
• culturing the cells in the sample in a culture medium comprising a T cell mitogen which is an antibody or fragment thereof and interleukin-15, in the absence of interleukin-4 and in the absence of feeder cells.