Biological pesticide fungus effective against multiple different species of insect
OwnerJohn Thomas Sigurdsoninventor
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Abstract
Compositions and methods for insect control are provided. In one example, a method of producing a mycoinsecticide composition comprises: preparing a starter culture to grow a strain of B. bassiana fungus; freezing and storing the prepared starter culture to obtain a fungus mixture; treating the stored fungus mixture to obtain a fungus liquid containing the grown B. bassiana fungus; treating and/or formulating the fungus liquid to obtain the mycoinsecticide composition; and packaging the mycoinsecticide composition. The B. bassiana fungus is preferably of the strain JTDRL-RC5A-HSSE deposited under NRRL Number 67768.
Claims21
1-26. (canceled)
A method of producing a mycoinsecticide composition, the method comprising:
- preparing a starter culture to grow a strain of Beauveria bassiana fungus by adding Beauveria bassiana fungus to a vessel and allowing the fungus to grow in the vessel at a temperature of about 60 to 80° F.;
- freezing and storing the prepared starter culture to obtain a fungus mixture;
- treating the stored fungus mixture to obtain a fungus liquid containing the grown Beauveria bassiana;
- treating and/or formulating the fungus liquid to obtain the mycoinsecticide composition;
- and
- packaging the mycoinsecticide composition.
A method of producing a mycoinsecticide composition, the method comprising:
- adding water to a cereal grain comprising rice and barley in a vessel and allowing the water to soak the cereal grain;
- adding Beauveria bassiana fungus and a glucose source to produce a fungus mixture having a glucose content of 4 to 6%, based on total weight of the fungus mixture;
- allowing the fungus mixture to grow in the vessel for 10 to 30 days at a temperature of 70 to 80° F.;
- freezing the fungus mixture a temperature of −22 to 0° F.;
- thawing and washing the fungus mixture with water;
- straining the resulting fungus liquid and storing the fungus liquid in a container;
- adding molasses to raising the total glucose content to 6 to 8%;
- adding fertilizer to the fungus liquid;
- and
- bubbling and stirring the fungus liquid in the container for 10 to 60 days.
Dependencies are inferred from the claim text.